Inflammatory bowel disease is one of the most important risk factor in the development of colon cancer. Various inflammatory mediators, oxidative stress and abnormal glycosaminoglycans are responsible for the development of inflammatory bowel disease. Present study was undertaken to evaluate the effect of alcoholic extract of Gardenia gummifera on experimentally induced inflammatory bowel disease in rats. Wistar rats of either sex (250-300gm) were treated for 7 days with alcoholic extract of Gardenia gummifera (100mg/kg, 250mg/kg and 500mg/kg) prior to administration of DNBS (2,4-dinitrobenzenesulfonic acid). After 7 days DNBS was given intrarectally to each animals and treatment was continued for 21 days. Body weight, food intake, water intake, malondialdehyde (MDA), superoxide dismutase (SOD), myeloperoxidase (MPO), nitric oxide (NO), mast cell protection, colon weight, colon length and histopathology based grading system like disease activity index, colon mucosa damage index were carried out after 21 days. The rats treated with 5-aminosalicylic acid were included as a standard. The rats treated with alcoholic extract of Gardenia gummifera and 5-aminosalicylic acid showed lower level of oxidative enzymes like malondialdehyde (MDA), myeloperoxidase (MPO), nitric oxide (NO) and higher of protective enzyme like superoxide dismutase (SOD) as compared to model control groups (DNBS treated). Alcoholic extract of Gardenia gummifera and standard (5-ASA) produced significant mast cell protection as well as significant protection in histopathology as compared to alone DNBS treated animals.
Loading....